Sammendrag
The aim of this study was to investigate the effect of TiO2 scaffold (SC) coated with an
alginate hydrogel containing a proline-rich peptide (P2) on osteoblast proliferation and
differentiation in vitro. Peptide release was evaluated and a burst release was observed
during the first hours of incubation, and then progressively released overtime. No changes
were observed in the cytotoxicity after 48h of seeding MC3T3-E1 cells on the coated and
uncoated TiO2 SC. The amount of cells after 7 days was higher on uncoated TiO2 SC than
on alginate-coated TiO2 SC, measured by DNA content and SEM imaging. In addition,
while lower expression of integrin beta1 was detected for alginate-coated TiO2 SC at this
time point, similar gene expression was observed for other integrins, fibronectin-1, and
several osteoblast differentiation markers. After 21 days, gene expression of integrin
beta3, fibronectin-1, osterix and collagen-I was increased in alginate-coated compared to
TiO2 SC. Moreover, increased gene expression of integrin alpha8, bone morphogenetic
protein 2, interleukin-6 and collagen-I was found on P2 alginate-coated TiO2 SC compared
to alginate-coated TiO2 SC. In conclusion, our results indicate that alginate-coated TiO2
SC can act as a matrix for delivery of proline-rich peptides increasing osteoblast
differentiation.
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