Cristin-prosjekt-ID: 455237
Sist endret: 1. desember 2017 09:46
Cristin-prosjekt-ID: 455237
Sist endret: 1. desember 2017 09:46
Prosjekt

PsyXpress: Development of a new bacterial host for efficient expression of cold-adapted enzymes

prosjektleder

Peik Haugen
ved Institutt for kjemi ved UiT Norges arktiske universitet

prosjekteier / koordinerende forskningsansvarlig enhet

  • Fakultet for naturvitenskap og teknologi ved UiT Norges arktiske universitet

Finansiering

  • TotalbudsjettNOK 10.329.000
  • Norges forskningsråd

    • Prosjektkode: 234541

Klassifisering

Vitenskapsdisipliner

Bioteknologi

Emneord

Proteinsyntese • Bakterier

Kategorier

Prosjektkategori

  • Anvendt forskning

Kontaktinformasjon

Telefon
77645288
Sted
Peik Haugen

Tidsramme

Avsluttet
Start: 1. juli 2014 Slutt: 31. desember 2017

Beskrivelse Beskrivelse

Tittel

PsyXpress: Development of a new bacterial host for efficient expression of cold-adapted enzymes

Populærvitenskapelig sammendrag

Cold-adapted enzymes are very challenging to produce in standard bacterial production hosts because here they typically form inactive aggregates. This challenge represents perhaps the biggest bottleneck for further developments of new enzyme products from marine bioprospecting.

Development of improved expression systems for enzymes with extreme properties could potentially have large impact on national and international biotech industry because such enzymes are highly efficient at low temperatures, and can therefore contribute to more environmentally friendly industrial processes and better quality on industrial products.

The main objective of PsyXpress is to develop a bacterium, which is naturally adapted to extreme environmental conditions, for efficient production of cold-adapted enzymes.

We have recently identified a marine bacterium as a very interesting candidate for further commercial developments. The most important reasons are that the bacterium grows well at low temperatures, it grows fast and to high densities, it is sensitive to a number of commonly used antibiotics, expression of enzymes can be induced simply by adding a synthetic chemical, the bacterial genome is known, and the its genome can be manipulated in a site-specific fashion using
standard genetic tools.

We expect that the end product of this project will be a new bacterial strain that will pave the way for more efficient production of enzymes adapted to extreme conditions. There is currently a great demand for such a product on the market, and several companies could have the system as a product in their portfolio, either in the form of commercial kits with live cells and reagents or as service where cloning and expression is done by the provider.

prosjektdeltakere

prosjektleder

Peik Haugen

  • Tilknyttet:
    Prosjektleder
    ved Institutt for kjemi ved UiT Norges arktiske universitet

Miriam Grgic

  • Tilknyttet:
    Prosjektdeltaker
    ved Institutt for kjemi ved UiT Norges arktiske universitet

Jenny Johansson Söderberg

  • Tilknyttet:
    Prosjektdeltaker
    ved Institutt for kjemi ved UiT Norges arktiske universitet
1 - 3 av 3

Resultater Resultater

Development of a novel expression host for challenging enzymes.

Söderberg, Jenny Johansson; Haugen, Peik; Grgic, Miriam. 2017, BIOPOSP_17. UITVitenskapelig foredrag

Development of a novel expression host for challenging enzymes.

Söderberg, Jenny Johansson; Haugen, Peik; Grgic, Miriam. 2017, BIOPOSP_17. UITPoster

Development of a new bacterial host for efficient expression of cold-adapted enzymes.

Haugen, Peik. 2015, 3rd annual optimalization workshop 2015. UITVitenskapelig foredrag

A novel expression system specialized for psychrophilic enzymes.

Söderberg, Jenny Johansson; Haugen, Peik. 2015, Bioprosp 2015. UITPoster

A novel expression system specialized for psychrophilic enzymes.

Grgic, Miriam; Söderberg, Jenny Johansson; Haugen, Peik. 2016, The 52nd NBS contact meeting. UITPoster
1 - 5 av 5