Cristin-resultat-ID: 1506918
Sist endret: 29. mai 2018 13:09
NVI-rapporteringsår: 2017
Resultat
Vitenskapelig artikkel
2017

Viruses on the menu: The appendicularian Oikopleura dioica efficiently removes viruses from seawater

Bidragsytere:
  • Janice Lawrence
  • Joachim Paul Töpper
  • Elzbieta Anna Petelenz-Kurdziel
  • Gunnar Bratbak
  • Aud Larsen
  • Eric Thompson
  • mfl.

Tidsskrift

Limnology and Oceanography
ISSN 0024-3590
e-ISSN 1939-5590
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2017
Publisert online: 2017
Trykket: 2018
Volum: 63
Sider: S244 - S253
Open Access

Importkilder

Scopus-ID: 2-s2.0-85043427102

Klassifisering

Vitenskapsdisipliner

Zoologiske og botaniske fag

Finansiering

  • Norges forskningsråd

    • Prosjektkode: 225956
    • Prosjektkode: 275710
  • Andre

    • Prosjektkode: NSERC

Beskrivelse Beskrivelse

Tittel

Viruses on the menu: The appendicularian Oikopleura dioica efficiently removes viruses from seawater

Sammendrag

Appendicularians are planktonic marine tunicates with elaborate filter-feeding houses that can efficiently trap particles as small as 0.2 lm. While marine viruses are seldom considered outside their role in disease transmission, we conducted a controlled laboratory experiment to determine if the appendicularian Oikopleura dioica can trap and ingest the Emiliania huxleyi virus (EhV; 160–180 nm diameter). Removal and retention of EhV during 2.5 h and overnight incubations at 158C were measured using flow cytometry and quantitative polymerase chain reaction specific for the mcp gene of EhV. The fate of retained EhV was tested by quantifying EhV DNA in three biological compartments: house-trapping, ingestion/digestion, and defecation. Clearance rates for EhV varied from approximately 2 mL ind21 d21 to 50 mL ind21 d21, with highest rates for 4–5 d-old animals. EhV particles were cleared by O. dioica at rates similar to those reported for larger food particles, with mean clearance rates in the 2.5 h incubations ranging from approximately 2 mL ind21 d21 to 50 mL ind21 d21. This demonstrates efficient virus removal by O. dioica and a previously overlooked link between the microbial loop and the classical marine food web. EhV DNA was readily detectable above background levels in O. dioica houses, gut contents, and faecal pellets, suggesting that appendicularian houses and faecal pellets may contribute to the dispersal of viruses. Furthermore, clearance of EhV and presumably other viruses by O. dioica may be a significant sink for viruses and thus an important factor in regulating the population dynamics of viruses and their hosts.

Bidragsytere

Janice Lawrence

  • Tilknyttet:
    Forfatter
    ved University of New Brunswick

Joachim Paul Töpper

  • Tilknyttet:
    Forfatter
    ved NINA Bergen ved Norsk institutt for naturforskning

Elzbieta Anna Petelenz-Kurdziel

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen

Gunnar Bratbak

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen

Aud Larsen

  • Tilknyttet:
    Forfatter
    ved Uni Research Miljø ved NORCE Norwegian Research Centre AS
  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen
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